Superior sensitivity——Down to 5 copies/ ul

The high sensitivity enables the detection of low RNA input. 

※Reference Material Producer：Shanghai Institute of Metrology and Testing Technology

Faster & Higher cDNA synthesis temperature

The optimal temperature for reverse transcriptase is 55℃ in this system. Higher temperature facilitates the amplification of templates with secondary structure and high GC contents.The RT reaction can be finished within 10 mins. NuHi’s mix can save 14-34 mins per run comparing with other EUA Mix.

High specificity

It is common for nonspecific binding to occur when the amplification reaction is set up at room temperature. Primers can bind to each other, forming primer–dimers. During amplification cycles, primer–dimers can be extended to produce nonspecific products.
However, NuHi’s mix adopted the innovated two-phase hot-start mechanism. The RT activity is suppressed below 37℃ and generates no primer-dimers, which means the specificity can be enhanced greatly.

※Activity comparison between normal  RT enzyme and hot-start RT enzyme  at 37 ℃

Multiplex detection

Capable of detecting 4-5 different genes within one assay.

Anti-contamination

Adding DUTP and UNG enzyme into system to realize the decontamination at room temperature. Compare to the system without UNG, the anti-contamination system shows no loss in PCR efficiency.