UNG enzyme

NuHi® UNG (thermal version)   catalog#: NH9003     size: 200U/ul

NuHi® E.coli UNG   catalog#: NH9006     size: 200U/ul


  • Overview
  • Downloads
  • FAQ

Product Description

PCR is an extremely sensitive amplification technique. A small amount of foreign DNA contamination will be amplified together with the target DNA template. The Ministry of Health has clearly stipulated that all PCR reagents used for clinical testing should have UNG enzyme technology to prevent aerosol contamination.

The working principle of UNG enzyme is to selectively hydrolyze and break the uracil glycosidic bond of double-stranded or single-stranded DNA containing dU. Replace dTTP with dUTP, so that all PCR products contain dU DNA strands. Add an incubation step before the PCR, and the UNG enzyme can degrade the uracil bases in the existing U-DNA contaminants in the reaction system, and under the subsequent denaturation conditions, the DNA strands will break, eliminating the aerosol pollution. Amplification to ensure the specificity and accuracy of the amplification results. The UNG enzyme is inactivated at a high temperature of 95℃ at the beginning of PCR, and will not degrade the newly amplified product U-DNA.


Key Features

NuHi® UNG (thermal version)

High activity at 20-40℃, inactivation at 95℃ for 2min, suitable for PH 7.0-8.5, most suitable for PH 7.5, 50mmol/L NaCl.


NuHi® E.coli UNG

High activity at 50℃, inactivation at 95℃ for 2min, suitable for PH 7.0-8.5, most suitable for PH 7.5, 50mmol/L NaCl.